TABLE 1.
Susceptibility profiles of K. pneumoniae strains and their transconjugants (TCs)/transformants (TFs) along with transmissibility of blaOXA-181-like and genotypic characterization of TCs/TFs established with PCR-based techniques
| Strain ID | MIC (mg/liter):a |
Resistance genes present/transferredb | Insertion sequence (IS) element | Plasmid type | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AN | CN | AT | CT | FX | CI | IP | ETP | MP | CO | TGC | PP | PTZ | ||||
| EN5153 (Kp1) | 8 | 96 | >256 | >256 | 48 | 4 | >32 | >32 | 12 | 1 | 0.75 | >256 | 96 | blaCTX-M-15, blaTEM-1B, blaSHV-1, blaOXA-1, blaOXA-181, oqxA, oqxB, aac(6′)-Ib-cr | Truncated ISEcp1 (167 bp) | IncFIIK, IncFIB(K), IncFIB (pQil), ColKP3 |
| Kp1 TF2 | 3 | 0.5 | 0.094 | 0.38 | 12 | <0.002 | 8 | 1 | 0.38 | 0.25 | 0.125 | 128 | 48 | blaOXA-181 | ND | ColKP3 |
| EN5172 (Kp2) | >256 | >1,024 | 48 | >256 | 6 | 32 | 16 | >32 | 2 | 1 | 0.38 | >256 | >256 | blaCTX-M-15, blaTEM-1B, blaOXA-1, blaOXA-181, aac(6′)-Ib, qnrB, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 303 bp of its RTE Ext present | IncFIIK, IncFII, ColKP3 |
| Kp2 TF2 | 4 | 0.5 | 2 | 2 | 6 | <0.002 | 4 | 12 | 1 | 0.5 | 0.125 | 128 | 96 | blaOXA-181 | ND | ColKP3 |
| Kp2 TF3 | 2 | 0.38 | 0.19 | 0.5 | 4 | <0.002 | 4 | 6 | 1 | 0.125 | 0.125 | 128 | 96 | blaTEM-1B, blaOXA-181 | ND | ColKP3, IncFII |
| EN5199 (Kp3) | >256 | >1,024 | >256 | >256 | >256 | >32 | 16 | >32 | >32 | 1 | 1 | >256 | >256 | blaCTX-M-15, blaTEM-1B, blaSHV-28, blaOXA-1, blaOXA-9, blaNDM-5, blaOXA-232, rmtB, aac(6′)-Ib, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 335 bp of its RTE Ext present | IncR, IncFIIK, IncFII, IncFIB (K), IncFIA (HI1), ColKP3 |
| Kp3 TC1 | >256 | >1024 | 16 | 96 | 48 | >32 | >32 | 32 | 24 | 0.5 | 0.19 | >256 | >256 | blaNDM-5, rmtB | ND | IncFII |
| Kp3 TC4 | >256 | >1,024 | 16 | 96 | 48 | >32 | >32 | 32 | 24 | 0.5 | 0.75 | >256 | >256 | blaCTX-M-15, blaTEM-1B, blaNDM-5, blaOXA-232, rmtB | ND | ColKP3, IncR, IncFII |
| EN5213 (Kp4) | >256 | >1,024 | >256 | >256 | 12 | >32 | 16 | >32 | 3 | 1 | 1 | >256 | >256 | blaCTX-M-15, blaSHV-28, blaOXA-1, blaOXA-181, aac(6′)-Ib, qnrB, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 339 bp of its RTE Ext present | IncFIIK, ColKP3 |
| Kp4 TF1 | 2 | 0.25 | 0.19 | 0.75 | 3 | <0.002 | 16 | 32 | 3 | 0.125 | 0.19 | >256 | >256 | blaOXA-181 | ND | ColKP3 |
| EN5218 (Kp5) | 32 | 2 | 32 | 256 | 4 | >32 | 8 | >32 | 1 | 1 | 1 | >256 | >256 | blaCTX-M-15, blaTEM-1A, blaSHV-28, blaOXA-1, blaOXA-9, blaOXA-181, aac(6′)-Ib, qnrB1, oqxA, oqxB, aac(6′)-Ib-cr | NF | IncFIIK, IncFII |
| Kp5 TC2 | 32 | 3 | 32 | 32 | 1 | >32 | 4 | 0.5 | 0.032 | 0.5 | 1 | >256 | >256 | blaCTX-M-15, blaSHV-28, blaOXA-181, aac(6′)-Ib-cr, qnrB, oqxA, aac(6′)-Ib-cr | ND | IncFIIK |
| EN5275 (Kp6) | >256 | >1,024 | >256 | >256 | >256 | >32 | 32 | >32 | >32 | 0.25 | 0.5 | >256 | >256 | blaCTX-M-15, blaTEM-1B, blaSHV-190, blaCMY-4, blaOXA-232, armA, rmtF, aac(6′)-Ib-Hangzhou, qnrB1, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but ∼128 bp of its RTE Ext present | Col4401I, Col4401II, ColKP3, IncA/C2, IncFIIK, IncX3, IncFIB(pQil) |
| Kp6 TF1 | 2 | 1.5 | 0.5 | 0.5 | 32 | 0.006 | 8 | 8 | 1 | 0.5 | 1 | >256 | >256 | blaOXA-232 | ND | ColKP3 |
| EN5280 (Kp7) | >256 | >1,024 | >256 | >256 | >256 | >32 | 32 | >32 | >32 | 0.5 | 0.38 | >256 | >256 | blaCTX-M-15, blaSHV-11, blaCMY-4, blaOXA-232, armA, aac(6′)-Ib, qnrB1, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 335 bp of its RTE Ext present | IncA/C, IncFIIK, IncX3, ColKP3 |
| Kp7 TF1 | 2 | 0.25 | 0.125 | 1 | 24 | <0.002 | 8 | 6 | 1.5 | 0.125 | 0.19 | >256 | >256 | blaOXA-232 | ND | ColKP3 |
| EN5338 (Kp8) | >256 | >1,024 | >256 | >256 | >256 | >32 | >32 | >32 | >32 | 0.5 | 1 | >256 | >256 | blaCTX-M-15, blaTEM-1B, blaSHV-28, blaOXA-232, rmtF, aac(6′)-Ib-Hangzhou, oqxA, oqxB, qnrS1 | ISEcp1 absent but ∼320 bp of its RTE Ext present | ColKP3, IncFIA, IncHI1B, IncFIB (Mar), IncFIB (pQil), IncFIIK, IncFII (pAMA1167-NDM-5) |
| Kp8 TF1 | 3 | 0.25 | 0.5 | 0.75 | 16 | 0.16 | 8 | 16 | 1.5 | <0.25 | 0.38 | >256 | >256 | blaOXA-232 | ND | ColKP3 |
| EN5339 (Kp9) | >256 | >1,024 | >256 | >256 | >256 | >32 | >32 | >32 | >32 | 64 | 1 | >256 | >256 | blaCTX-M-15, blaTEM-1A, blaOXA-1, blaOXA-9, blaNDM-5, blaOXA-181, rmtB, aac(6′)-Ib, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 335 bp of its RTE Ext present | ColKP3, IncFIA (HI1), IncFIB (K), IncFIB (pKPHS1), IncR, IncFIIK, IncFII |
| Kp9 TC2 | >256 | >1,024 | 32 | >32 | 64 | >32 | >32 | >32 | 6 | 0.5 | 1 | >256 | >256 | blaNDM-5, rmtB, oqxA, oqxB | ND | IncFII |
| Kp9 TC3 | >256 | >1,024 | 32 | >32 | 96 | >32 | >32 | >32 | 12 | 0.5 | 1 | >256 | >256 | blaNDM-5, blaOXA-181, rmtB, oqxA, oqxB | ND | IncR, IncFII, ColKP3 |
| EN5340 (Kp10) | >256 | >1,024 | >256 | >256 | >256 | >32 | >32 | >32 | >32 | 64 | 0.75 | >256 | >256 | blaCTX-M-15, blaTEM-1A, blaOXA-1, blaOXA-9, blaNDM-5, blaOXA-181, rmtB, aac(6′)-Ib, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 335 bp of its RTE Ext present | ColKP3, IncFIA (HI1), IncFIB, IncR, IncFIIK, IncFII |
| Kp10 TC1 | >256 | >1,024 | 48 | >32 | 96 | >32 | >32 | 24 | 32 | 0.25 | 1 | >256 | >256 | blaNDM-5, blaOXA-181, oqxA, oqxB | ND | ColKP3, IncFII, IncR |
| EN5343 (Kp11) | >256 | >1,024 | >256 | >256 | >256 | >32 | >32 | >32 | >32 | 64 | 1 | >256 | >256 | blaCTX-M-15, blaTEM-1A, blaOXA-1, blaOXA-9, blaNDM-5, blaOXA-181, rmtB, aac(6′)-Ib, oqxA, oqxB, aac(6′)-Ib-cr | ISEcp1 absent but 335 bp of its RTE Ext present | ColKP3, IncFIA (HI1), IncFIB, IncR, IncFIIK, IncFII |
| Kp11 TC1 | >256 | >1,024 | 32 | >32 | 32 | >32 | >32 | 24 | >32 | 0.5 | 0.25 | >256 | >256 | blaNDM-5, rmtB, oqxA, oqxB | ND | IncFII |
| Kp11 TC2 | >256 | >1,024 | 48 | >32 | 48 | >32 | >32 | 12 | 24 | 0.5 | 1 | >256 | >256 | blaNDM-5,blaOXA-181, rmtB, oqxA, oqxB | ND | ColKP3, IncR, IncFII |
a
Abbreviations: TC, transconjugant; TF, transformant; AN, amikacin; CN, gentamicin; AT, aztreonam; CT, cefotaxime; FX, cefoxitin; CI, ciprofloxacin; IP, imipenem; ETP, ertapenem; MP, meropenem; CO, colistin; TGC, tigecycline; PP, piperacillin; PTZ, piperacillin-tazobactam; ND, not done; NF, not found; RTE Ext, right-end extremity of ISEcp1.
b
Transferred carbapenem-resistant genes have been boldfaced.