FIG 1.

AM.P2, a Podoviridae phage isolated from wastewater, produces plaques against PAO1. (A) Isolated phage AM.P2 against host strain PAO1, plated by a double-layer agar method to visualize individual plaques. AM.P2 produces large plaques (5-mm diameter) against PAO1 with clear centers and turbid edges when plated with 0.7% soft agar. (B) Cryo-EM raw image of the AM.P2 phage with the capsid and the tail. (C) Image of several AM.P2 phage particles attached to a bacterial cell-like structure. (D) Restriction digestion of AM.P2 nucleic acid with EcoRI, BamHI, and HindIII.