. 2021 Jan 20;6(1):e01079-20. doi: 10.1128/mSphere.01079-20

TABLE 2.

Catalytic properties of putative crotonyl-CoA hydratases Msed_0566^d

Substrate	V_max (U mg⁻¹ protein)		K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a
Substrate	Measured (65°C)	Extrapolated to 75°C	K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a
Crotonyl-CoA	1.37 ± 0.15	2.74 ± 0.30	0.08 ± 0.03	19
(S)-3-Hydroxybutyryl-CoA	0.07^b^,^c	0.7	NA	NA
3-Hydroxypropionyl-CoA	0.04^b^,^c	0.4	NA	NA
Acryloyl-CoA	1.34 ± 0.04	2.68 ± 0.08	0.037 ± 0.002	39
Methacrylyl-CoA	0.26 ± 0.02	0.52 ± 0.04	0.14 ± 0.03	2
(E)-2-Octenoyl-CoA	2.61 ± 0.34	5.22 ± 0.68	0.18 ± 0.06	16

k_cat was calculated for the activities at 75°C.

Activity was measured at 42°C.

Specific activity.

The V_max values are normalized to 75°C based on the assumption that a 10°C rise in temperature doubles the reaction rate. NA, not applicable.