. 2021 Jan 20;6(1):e01079-20. doi: 10.1128/mSphere.01079-20

TABLE 4.

Catalytic properties of 3-hydroxypropionyl-CoA dehydrases/crotonyl-CoA hydratases Msed_2001 and Nmar_1308 as well as homologous enoyl-CoA hydratase Slip_2089 from S. lipocalidus^d

Substrate	Msed_2001				Nmar_1308			Slip_2089
	V_max (U·mg⁻¹ protein)		K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a	V_max (U·mg⁻¹ protein)	K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a	V_max (U·mg⁻¹ protein)		K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^c
	Measured (65°C)	Extrapolated to 75°C	K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a	V_max (U·mg⁻¹ protein)	K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^a	Measured (42°C)	Extrapolated to 55°C	K_m (mM)	k_cat/K_m (s⁻¹ mM⁻¹)^c
Crotonyl-CoA	2,461 ± 135	4,922 ± 270	0.08 ± 0.01	31,621	3,793 ± 111	0.45 ± 0.03	4,251	18 ± 1	44 ± 2	0.16 ± 0.02	143
(S)-3-Hydroxybutyryl-CoA	294 ± 11^b	2,896 ± 108	0.034 ± 0.004	43,776	1,515 ± 92	0.36 ± 0.06	2,122	42 ± 5	103 ± 12	1.31 ± 0.31	41
3-Hydroxypropionyl-CoA	103 ± 5^b	1,014 ± 49	0.16 ± 0.02	3,257	38 ± 2	0.17 ± 0.03	113	0.45 ± 0.03	1.11 ± 0.07	0.35 ± 0.06	2
Acryloyl-CoA	13,532 ± 572	27,064 ± 1,144	0.14 ± 0.01	99,354	11,391 ± 392	0.71 ± 0.07	8,091	43 ± 2	106 ± 5	0.40 ± 0.05	138
Methacrylyl-CoA	804 ± 43	1,608 ± 86	2.19 ± 0.23	377	116 ± 13	0.83 ± 0.24	70	0.38 ± 0.03	0.94 ± 0.07	1.01 ± 0.22	0.5
(E)-2-Octenoyl-CoA	1,842 ± 70	3,684 ± 140	0.048 ± 0.005	39,446	47 ± 2	0.12 ± 0.02	198	92 ± 5	227 ± 12	0.21 ± 0.04	562

k_cat was calculated for the activities at 75°C.

Activity was measured at 42°C.

k_cat was calculated for the activities at 55°C.

The V_max values are normalized to 75°C based on the assumption that a 10°C rise in temperature doubles the reaction rate.