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. 2021 Jan 19;23(3):213. doi: 10.3892/mmr.2021.11852

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Copyright: © Luo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of miR-140-5p knockdown and ox-LDL treatment on VSMC viability, migration, invasion and apoptosis. (A) VSMCs were identified via immunofluorescence (magnification, ×200). (B) Transfection efficiency of miR-140-5p inhibitor. (C) miR-140-5p and ROBO4 expression levels in miR-140-5p inhibitor-transfected and ox-LDL-treated VMSCs. (D) ROBO4 protein expression levels in miR-140-5p inhibitor-transfected and ox-LDL-treated human VSMCs (E) Bcl-2 and MMP2 expression levels were measured by reverse transcription-quantitative PCR. Human VSMC (F) viability, (G) migration, invasion (magnification, ×200) and (H) apoptosis following miR-140-5p inhibitor transfection and ox-LDL treatment. *P<0.05, **P<0.01, ***P<0.001 vs. NC or control; ^#P<0.05, ^##P<0.01 vs. ox-LDL + NC. miR, microRNA; ox-LDL, oxidized-low density lipoprotein; VSMC, vascular smooth muscle cell; ROBO4, roundabout guidance receptor 4; MMP2, matrix metallopeptidase 2; α-SMA, α-smooth muscle actin; NC, negative control; OD, optical density.