Figure 3.

Investigation of miR-140-5p binding to ROBO4 mRNA in VSMCs. (A) The predicted binding site between miR-140-5p and the 3′-UTR sequences of ROBO4 mRNA. (B) Verification of miR-140-5p binding to the 3′-UTR sequences of ROBO4 mRNA in human VSMCs. (C) Expression of ROBO4 in VSMCs following transfection with miR-140 mimic. **P<0.01, vs. NC. miR, microRNA; ROBO4, roundabout guidance receptor 4; VSMC, vascular smooth muscle cell; 3′-UTR, 3′-untranslated region; WT, wild-type; MUT, mutant; NC, negative control; ox-LDL, oxidized-low density lipoprotein.