FIG 1.

Characterized viruses detected in enriched and unenriched wastewater metatranscriptomes. The relative abundances of viruses with eukaryotic hosts in the RefSeq database as a percentage of total sequencing reads derived from the sample in Amicon ultrafiltration (viral fractionation) (a) and total RNA column and milk of silica samples (b). All samples were enriched with the Illumina respiratory virus panel. (c) Relative abundances of RefSeq viruses in unenriched metatranscriptomics (left) and the same samples after oligonucleotide enrichment with the Illumina respiratory virus panel. (d) The relationship between the quantity of viral genome copies in 40 μl of purified RNA and SARS-CoV-2 genome completeness (measured in breadth of coverage) for each sample. Samples are colored by extraction methodology, and the size of the point corresponds to the mean SARS-CoV-2 depth of coverage.