Skip to main content
. Author manuscript; available in PMC: 2021 Jan 29.
Published in final edited form as: Methods Mol Biol. 2021;2236:115–127. doi: 10.1007/978-1-0716-1060-2_11

Fig. 1.

Fig. 1

Flow cytometric determination of reactive oxygen species by MDSC in response to M. tuberculosis. Sorted MDSC and non-MDSC (HLA-DRhi) are infected with GFP-M. tuberculosis Erdman and incubated with CellROX for 30 min. Cells are stained with Live/Dead stain, formaldehyde fixed, and analyzed using flow cytometer. The gating strategy is shown