HCO3− transport in a β-cyanobacterial cell starts at the plasma membrane, where the transporters BicA, SbtA, and BCT1 are located. After being concentrated in the cytoplasm, HCO3− diffuses into the carboxysome, likely via pores in the shell protein CcmK4. Inside the carboxysome, carbonic anhydrases convert HCO3− into CO2, which can then be fixed by Rubisco clustered there. CO2 that diffuses into the cytoplasm or leaks out of the carboxysome is converted to HCO3− by the NDH-13 and NDH-14 complexes.