Figure 7. PRRSM classifies apo and bound secondary structures in prequeuosine-1 (PreQ1) riboswitch.

A. Left: PreQ riboswitch secondary structure in the unbound and bound state. Right: Bound structure of PreQ1 (PDB 2L1V) with highlighted sites of BFU fluorophore insertion (U9-red, U11-blue, U14-green) and PreQ1 ligand (orange). B. PCA plot of the U9, U11, and U14 modified RNA in the absence (w/o Lig) and presence (w Lig) of PreQ1 ligand. All constructs were predicted to be the correct structure in both the unbound and bound states under standard conditions (10 mM NaH₂PO₄, 25 mM NaCl, 4 mM MgCl₂, 0.5 mM EDTA, pH 7.3 at 25 °C. Reprinted with permission from Ref. 112. Copyright 2019 American Chemical Society.