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. 2021 Jan 19;17(1):e1009310. doi: 10.1371/journal.pgen.1009310

Table 3. RVD variants of the original and adapted dTALEs.

dTALE dTALE RVD Parental dTALE Binding affinity score to the LOB1 promoterA Number of infection cycles for adaptation Found after 30 infection cyclesC Detected in replicateD
1 2
ScoreB Best possible scoreB
dTALEWTLOB1 NI NG NI NI NI HD HD NG HD NG NG NG NG NN HD HD NG NG NN NAE 7.35 5.34 NA NA NA
dTALELBM1 NI NH NG NN NI NI NG NG NI NH NG NG NG NN HD HD NG NG NN NA -F 5.27 NA YES YES YES
dTALELBM2 NG NI NI NI NI HD HD NG HD NG NG NG NG NN HD HD NG NG NN NA 12.87 5.34 NA NO NO NO
dTALELB2A1 NGNGNININIHDHDNGHDNGNGNGNGNNHDHDNGNGNN dTALELBM2 9.45 5.45 17 YES YES YES
dTALELB2A2 NINGNININIHDHDNGHDNGNGNGNGNN dTALELBM2 5.65 3.65 17 NO NO YES
dTALELBM3 NG NI NG NI NI HD HD NG HD NG NG NG NG NN HD HD NG NG NN NA 14.97 5.45 NA YES NO YES
dTALELB3A NGNINIHDHDNGHDNGNGNGNGNNHDHDNGNGNN dTALELBM3 9 5 9 YES YES NO
dTALELBM4 HD NG HD NI NG NG NG HD NI NI NG NG NG NN HD HD NG NG NN NA 21.66 5.45 NA YES YES YES
dTALELBM5 NI NG NI NI NI HD HD NG HD NG NG NI NI NN HD HD NG NG NN NA 14.17 5.14 NA YES YES NO
dTALELB5A NINGNININIHDHDNGHDNGNGNINGNNHDHDNGNGNN dTALELBM5 10.76 5.24 19 YES NO YES
dTALELBM6 HD HD NG NG NG HD NI NI NG HD NG NG NG NN HD HD NG NG NN NA 25.66 6.81 NA YES YES YES
dTALELBM7 NI NG NI NG NI NI NI NI HD HD NG NG NG NN HD HD NG NG NN NA 16.63 5.26 NA YES NO YES
dTALELB7A NINGNINGNININIHDHDHDHDHDNGNGNN dTALELBM7 8.86 3.58 28 YES YES NO

Note

A The binding affinity analysis was conducted in LOB1 variant A from sweet orange (Citrus × sinensis). The promoter region was set as the 1,000 bp sequence upstream of the transcriptional start site.

B According to the target finder tool provided by https://tale-nt.cac.cornell.edu/.

C A clone is defined as “detected” if the dTALEs or adapted TALEs were present in plasmids isolated from bacteria at cycle 30. Three independent clones per strain were isolated from Xcc pthA4:Tn5, introduced to E. coli and sequenced.

D Each experiment was conducted with two replicates marked as “1” and “2”. Data states whether the inducted dTALE was identified in each replicate in the duration of the experiment representing both the time of adaptation and the end of the experiment as cycle 30.

E NA: not applicable.

F Score is beyond cutoff.

Blue color indicates adapted TALEs.