Fig 2. The loss of htt rescues the dsh¹ MB axon outgrowth mutant phenotype.

(A-B) Anti-Fas2 staining reveals the absence of β lobe in a dsh¹ mutant brain (A), which is rescued by the loss of one copy of htt (B). (C-D) Anti-Fas2 staining reveals the loss of the two β lobes in an Appl^d dsh¹ mutant brain (C), which is rescued by the loss of one copy of htt (D). (E-F) Quantitation of the rescue of dsh¹ (E) and of Appl^d dsh¹ (F) phenotypes by htt-ko. n = number of MBs analyzed and *** P < 0.001 (Fisher exact test). All panels correspond to adult brains. The scale bar on panels A-D indicates 30 μm. Images are composite stacks to allow the visualization of axon trajectories along their entire length. Full genotypes: (A) w dsh¹. (B) w dsh¹;; htt-ko / +. (C) Appl^d w* dsh¹. (D) Appl^d w* dsh¹;; htt-ko / +. (E) top to bottom: w dsh¹. w dsh¹;; htt-ko / +. Appl^d w* dsh¹. Appl^d w* dsh¹;; htt-ko / +.