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. 2021 Jan 19;17(1):e1009287. doi: 10.1371/journal.pgen.1009287

Fig 4. htt interacts with Abl.

Fig 4

(A) The Appld mutant phenotype is enhanced in an Abl2/+ mutant background. (B) However, the Appld mutant phenotype is not modified in an Abl2 httint/+ + mutant background. (C) The Abl2/Abl1 mutant phenotype is partially rescued by the loss of one copy of htt as inferred by the increase of the wild-type (WT) MBs. (D) The absence of α and β lobe phenotype observed in UAS-Abl driven by OK107-GAL4 is strongly increased by the loss of one copy of htt (upper and middle bars). However, this phenotype is rescued by the overexpression of full-length htt (upper and lower bars). n = number of MBs analyzed, ** P<0.01 and *** P < 0.001. Significance was calculated by a Chi2 test for A-C and by a multiple comparison Fisher’s exact test (P = 3.4 10−28) followed by post-hoc Bonferroni’s multiple comparison correction (p-values = 6.3 10−12 and 0.0003 for D). All panels correspond to adult brains except for B which is from 48 hAPF brains. Full genotypes: (A) top to bottom: Appld w* / Y; c739-GAL4 UAS-mito-GFP / +. Appld w* / Y; c739-GAL4 UAS-mito-GFP / +; Abl2 / +. Appld w* / Y; c739-GAL4 UAS-mito-GFP / +; Abl2 FRT2A httint / +. (B) top to bottom: y w67c23;; Abl2 FRT2A / Abl1 FRT2A. y w67c23;; Abl2 FRT2A httint / Abl1 FRT2A. (C) top to bottom: y w67c23 / Y; UAS-Abl / +; UAS-FRT-y+-FRT / +; OK107-GAL4 / +. y w67c23 / Y; UAS-Abl / UAS-mCD8-GFP; httint / +; OK107-GAL4 / +. y w67c23 / Y; UAS-Abl, UAS-htt-fl-CTAP / +;; OK107-GAL4 / +. Note that UAS-FRT-y+-FRT and UAS-mito-GFP are used here as neutral UAS to adjust for 2 UAS sequences in the three different genotypes.