Table 1. Primers designed for amplification of the partial sequences of E. multilocularis cox1, nad2 and cob genes using nested PCR.
Primer | Sequence (5′-3′) (First amplification) | Sequence (5′-3′) (Second amplification) |
---|---|---|
cox1-F | GTGGTGTTGATTTTTTGATGTTT | AGCAGGTGTTTCTAGAGTTTTTAGT |
cox1-R | CCAAACGTAAACAACACTATAAAAGA | ACCCACCACAAAATAGGATCACT |
nad2-F | GTTGAGCTATGTAATAATGTGTGGAT | GCGTTGATTCATTGATACATTG |
nad2-R | AAATCTGTTGAATCTGCTACAACC | TAGTAAAGCTCAAACCGAGTTCT |
cob-F | GGGTATGGCTTTGTATTATGGTAGTT | GTTTAAACTGGTAGATTGTGGTTC |
cob-R | ATCACTCAGGCTTAATACTAACAGGAG | CTCCACAGTAGAAATCACCATCA |