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. 2021 Jan 29;17(1):e1009237. doi: 10.1371/journal.ppat.1009237

Fig 3. HBGA phenotyping by immunofluorescence antibody.

Fig 3

The first row is mouse mAb BG-2 (anti-A antibody) followed by Alexa Fluor 488 Goat anti-mouse IgG3 (1:1000, Thermo Fisher Scientific) stained green. The second row is the corresponding DAPI staining (blue) for the first row. The third row is mouse mAb BG-4 (anti-H antibody) followed by Alexa Fluor 488 Goat anti-mouse IgG3 (1:1000, Thermo Fisher Scientific) stained green and the fourth row is the corresponding DAPI staining for the third row. Thus, the left, middle, and right column represent A+H+, A+, and H+ PIEs, respectively.