Table 1. Oligonucleotides used for quantitative real-time PCR analysis.

N gene region*	Forward primer	Reverse primer	Probe
N2	TTACAAACATTGGCCGCAAA	GCGCGACATTCCGAAGAA	FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1
N3	GGGAGCCTTGAATACACCAAAA	TGTAGCACGATTGCAGCATTG	VIC-ATCACATTGGCACCCGCAATCCTG-BHQ2

*Sequences used are recommended for SARS-CoV-2 detection by Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. All oligonucleotides were synthesized with DNK-sintez (Russia). Sequences are presented in 5′-3′ direction. FAM, fluorescein; VIC, 2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein; BHQ1/2, Black Hole Quencher 1/2. The working concentration of each oligonucleotide is 500 nM.