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. Author manuscript; available in PMC: 2021 Jan 29.
Published in final edited form as: Neurobiol Aging. 2019 May 3;80:154–172. doi: 10.1016/j.neurobiolaging.2019.03.018

Figure 6. WD causes activation of phagocytosing myeloid cells.

Figure 6

(A, B) A representative image of the corpus callosum from an aged WD mouse showing myelin (MBP), myeloid cells (IBA1) and phagosome-containing myeloid cells (CD68). (C) There was a significant increase of IBA1+ cells in the corpus callosum of aged WD compared to aged chow mice (n≥7, **p= 0.0012). (D) Myelin-myeloid cell interactions were also significantly increased in aged WD compared to aged chow mice (n≥5, **p= 0.0035). (E–G) IMARIS was used to identify myeloid cells that were actively phagocytosing myelin. The image shows activated myeloid cells interacting with myelin visualized with anti-MBP (E). F and G are higher resolution images from the boxed region in E and show MBP (red) contacting myeloid cells (green). Labeling in purple shows the interactions between CD68+IBA1+ cells. Arrows (F, G) show MBP inside the cell body of the myeloid cells.