Fig. 4.

Relative gene expression of relevant Cyp enzymes in response to carcinogen treatment. FE1 cells were treated with indicated concentrations of acrylamide −S9 (A), PhIP −S9 (B) and 4-ABP −S9 (C) for 6 h. Controls were exposed to water (acrylamide) or 0.1% DMSO (PhIP and 4-ABP) only. Cyp1a1, 1a2, 1b1 and 2e1 expression was determined by RT-qPCR and the 2^−ΔΔCt method. Values are normalised to mRNA expression of the housekeeping gene GAPDH and are relative to the water (A) or DMSO (B and C) control (n > 3). Statistical analysis was performed by log2 transforming the data and analysis using a single-sample t-test with Bonferroni correction against the population control mean of 0 (*P < 0.05, **P < 0.01).