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Carcinogen concentrations used to treat FE1 cells for the FE1 cell mutagenicity assay based on cell viability (% of control) determined by crystal violet staining
| Cell viability (% of control) | Acrylamide (mM) | Glycidamide −S9 (mM) | PhIP (μM) | N-OH-PhIP −S9 (μM) | 4-ABP (μM) | N-OH-4-ABP −S9 (μM) | |||
|---|---|---|---|---|---|---|---|---|---|
| −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | ||||
| 100 | 2 | 0.5 | 1 | 25 | 0.625 | 1.25 | 100 | 100 | 1 |
| 80 | 3 | 1 | 1.5 | 50 | 1.25 | 2 | 200 | 300 | 1.5 |
| 60 | 4 | 2 | 2 | 100 | 2.5 | 2.5 | 300 | 400 | 3 |
| 40 | 8 | 3 | 2.5 | 200 | 2.75 | 4 | 500 | 500 | 3.5 |
| 20 | 10 | 4 | 3.5 | 250 | 5 | 5 | 550 | 520 | 4.5 |
