Skip to main content
. 2021 Jan 19;17(1):e1009226. doi: 10.1371/journal.ppat.1009226

Fig 5. MHV-ExoN(-) DVG junction sites display both WT-like patterns of sequence composition and multiple alterations in nucleotide frequency, revealing microhomology at junctions.

Fig 5

(A) Nucleotide composition was calculated as the percent adenosine (A), cytosine (C), guanine (G), and uracil (U) at each position in a 30-base pair region flanking DVG junction start and stop sites in MHV-WT (blue) and MHV-ExoN(-) (orange) infected monolayer RNA. The junction is labelled as a carat (^) and a solid red line with upstream positions numbered -30 to -1 and downstream positions +1 to +30. The expected nucleotide percentage was calculated based on the overall MHV genome and represented as a dashed black line. Each point represents a mean (N = 3) and error bars represent SEM. 2-way ANOVA with multiple comparisons corrected for false discovery rate (FDR) by the Benjamini-Hochberg method. * q < 0.05, ** q < 0.01, *** q < 0.001, **** q < 0.0001. (B) Distribution of microhomology overlaps in MHV-WT (blue) and MHV-ExoN(-) (orange) compared to an expected probability distribution (gray). The frequency of each overlap length is displayed as a mean (N = 3) and error bars represent SEM. See also S5 Fig.