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. 2021 Jan 19;17(1):e1009284. doi: 10.1371/journal.pgen.1009284

Fig 6. MAST2 regulates TFPI and SERPINE1 promoter activity.

Fig 6

(A). HEK293 cells were transfected with control siRNA or with the two MAST2 specific siRNA and MAST2 expression was analyzed by western blot 48 hours post transfection. The detection of β-actin attests for equal protein loading. (B). HEK293 cells were cotransfected with control siRNA or with the two MAST2 specific siRNA together with TFPI (left) or SERPINE1 (right) promoter reporter. 48 hours post transfection, the activity of the promoters was measured as described in Materials and Methods section. (C). HEK293 cells were transfected with empty vector (ev), wild type MAST2 or MAST2 Gln89 expression vector and MAST2 expression was analyzed by western blot 48 hours post transfection. The detection of β-actin attests for equal protein loading. (D). HEK293 cells were cotransfected wild type MAST2 or MAST2 Gln89 expression vector together with TFPI (left) or SERPINE1 (right) promoter reporter then the activity of the promoters was measured as in Fig 4B. Data are means ± Standard Deviation. Statistical analyses were made using Mann-Whitney. **p<0.01; ***p<0.001: significant vs control.