Table 5.
Potency Score, Chemical, and Carbonyl Burdens for E-Liquids
| E-Liquid a |
Assay
b
|
||||||
|---|---|---|---|---|---|---|---|
| NRU | ROS | TBARs | MN | Total Score c | Chemicals (mg/ml) d | Carbonyls (ng/Puff) e | |
| Unflavored | 1 | 0 | 0 | 5 | 6 | 0.46 | 101 |
| Arctic Blast | 0 | 1 | 2 | 0 | 3 | 8.20 | 2576 |
| Blue Pucker | 2 | 3 | 2 | 5 | 14 | 1.94 | 1232 |
| Jamestown | 2 | 1 | 1 | 8 | 12 | 0.35 | 186 |
| Love Potion | 2 | 8 | 6 | 1 | 17 | 3.12 | 1319 |
| Mardi Gras | 1 | 4 | 0 | 3 | 8 | 1.90 | 2171 |
| Midnight Splash | 0 | 3 | 4 | 2 | 9 | 0.88 | 4090 |
| Port Royale | 0 | 5 | 3 | 3 | 11 | 14.60f | 2156 |
| Tobacco Row | 2 | 4 | 2 | 4 | 10 | 4.35 | 690 |
| Tortuga | 0 | 5 | 0 | 2 | 7 | 9.69 | 424 |
| Uptown | 1 | 1 | 0 | 4 | 6 | 1.59 | 1930 |
a
All e-liquids contain 70%PG/30%VG.
b
One point for each exposure causing ≥20% cytotoxicity assessed by NRU, 1 point for each exposure when significant for ROS-Glo, TBARs, and MN (micronuclei) and 1 point when dose response was seen.
c
There was no correlation between chemicals (p = .96) or carbonyls (p = .62) and total score.
d
Total level of 30 chemical constituents previously assessed (Zhou et al., 2020).
e
Combined level of carbonyls: formaldehyde, acetaldehyde, and acrolein previously quantified from e-liquid vapor containing nicotine at 5 V (Zhou et al., 2020).
f
Menthol comprised 12.26 mg/ml of the chemical burden.