Figure 1.

A flow chart of the three-step search algorithm to approximate mutation loads within an autozygous segment to estimate the minimum length of an ROH. Step 1: Identification of ROH segments based on the predefined target tract (e.g., 1 to 2 Mb) and selection of animals with similar nontarget tract length. Step 2: Clustering the selected animals into HIGH and LOW groups based on the total length of their target tracts. Step 3: Estimation of the difference in the effect of two groups on the trait of interest and its associated significance level.