Skip to main content
. 2021 Jan 29;7(5):eabc2578. doi: 10.1126/sciadv.abc2578

Fig. 7. c-Myc induces neuronal repressor REST to mediate lineage conversion.

Fig. 7

(A) Expression of REST in NCI-H1963 cells with LacZ overexpression and NCI-H1963 with c-Myc overexpression (n = 3). (B) Protein expression of c-Myc and Rest, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells. (C) Protein expression of Rest and ASCL1, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells to overexpress Rest. (D) Protein expression of c-Myc, Rest, and ASCL1, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells genetically deleted for REST and overexpressed with either LacZ as control or c-Myc. (E) Protein expression of c-Myc, L-Myc, ASCL1, and Rest, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells treated with vehicle [dimethyl sulfoxide (DMSO)] or 10 μM Rest inhibitor (X5050) for 48 hours. (F) Rest ChIP-qPCR in NCI-H1963 c-Myc–overexpressed cells and LacZ-overexpressed cells (negative control) at the ASCL1 and MYCL loci with 7q31 and HOPX as negative control loci. One representative of three biological replicates is shown. (G) Protein expression of c-Myc, L-Myc, ASCL1, and Rest, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells treated with vehicle (DMSO) or 0.5 μM Notch inhibitor (DBZ) for 48 hours. (H) Protein expression of c-Myc, L-Myc, ASCL1, and Rest, as well as vinculin, as a loading control in genetically engineered NCI-H1963 cells treated with vehicle (DMSO) or 10 μM Notch inhibitor (DAPT) for 48 hours. (I) c-Myc ChIP-qPCR in NCI-H1963 c-Myc–overexpressed cells and LacZ-overexpressed cells (negative control) at the ASCL1 and REST loci with 7q31 and HOPX as negative control loci. One representative of three biological replicates is shown. (J) Graphical schematic representing role of c-Myc and L-Myc as lineage-determining factors in SCLC.