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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cell Metab. 2020 Nov 6;33(1):94–109.e8. doi: 10.1016/j.cmet.2020.10.002

Figure 2. G6PD renders immortalized murine and human cells tumorigenic in animals.

Figure 2.

(A–F) NIH3T3 (A–C) and BJ (D–F) cells harboring H-RasV12, G6PD, or control vector were subcutaneously injected into athymic nude mice. Shown are growth of tumors over time (A and D) and images (B and E) and weights (C and F) of tumors at the end point of experiment. (G and H) Tumors generated by H-RasV12- and G6PD-expressing cells were analyzed with immunohistochemistry for proliferative cells by Ki-67 staining (G) and for blood density by endothelial marker CD31 staining (H). Shown are representative images of Ki-67 staining (Left) and percentages of proliferating cells (right) (G), and representative images of CD31 staining (Left) and blood vessels density normalized by that of H-Rasv12 (right) (H). Scale bars, 40 μm in (G), 200 μm in (H).

Individual data and means ± SD are shown. ***P < 0.001.