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. 2021 Jan 25;31(2):283–296.e7. doi: 10.1016/j.cub.2020.10.025

Figure 5.

Figure 5

An Intact Ctf19cCCAN Is Required for Functional Outer Kinetochore Assembly in Meiosis I

(A and B) Abnormal kinetochore behavior in the absence of MCM21.

(A) Representative images of wild-type and mcm21Δ cells carrying Mtw1-tdTomato and Ndc80-GFP after release from prophase I arrest and imaged throughout meiosis. Time after release from prophase I is indicated.

(B) Scoring of Mtw1-tdTomato signal in (A). Cells showing kinetochore spreading in at least one time point during the time-lapse were included in the “spreading” category. n > 49 cells.

(C) Non-degradable ndc80(Δ2-28) does not rescue kinetochore function upon the loss of MCM21. Dsn1-tdTomato signal was scored in prophase I wild-type and mcm21Δ cells expressing either Ndc80-GFP or Ndc80(Δ2-28)-GFP. n > 56 cells.

See also Figure S5.