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. 2021 Jan 29;11:2636. doi: 10.1038/s41598-021-81760-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time RT-PCR validation of chimeric A/B sandards. Amplification curves for genes targeted in the CDC real-time RT-PCR test for SARS-CoV-2 (N1, N2, N3 and RP). (a) Dilution series showing amplification curves for standards A (red line) and B (blue line). (b) Amplification curves comparing the chimeric A/B standards with IDT positive (nCoVPC, purple line) and negative controls (HSRP30, green line). (c, d) Amplification curves comparing the chimeric A/B standards with positive COVID-19 cDNA and RNA patient samples (green line, n = 12).