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. 2021 Jan 29;4:127. doi: 10.1038/s42003-021-01647-8

Fig. 2. FANCD2 binds to CFS genes and prevents their instability in a transcription-dependent manner.

Fig. 2

a FHIT mRNA level detected by RT-qPCR in WT and FHIT-KO cells after control or FANCD2 siRNA transfection. b FRA3B instability in WT and FHIT-KO cells transfected with control or FANCD2 siRNA and treated with 0.3 µM APH. n = 4 (WT), n = 3 (FHIT-KO) independent experiments. **p = 0.0064 (WT_siFANCD2), **p = 0.0074 (FHIT-KO_siLacZ), **p = 0.0028 (FHIT-KO_siFANCD2). c FANCD2 ChIP followed by qPCR in wild-type (WT) and FHIT-KO cells treated or not with 0.3 µM APH. The results are expressed as the percentage of input. n = 5 (IMMP2L), n = 3 (WWOX, FHIT). *p = 0.026, **p = 0.0035. Error bars are standard error of the mean (SEM).