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. 2021 Jan 29;6:18. doi: 10.1038/s41541-021-00281-z

Fig. 1. OMVs derived from B. pseudomallei grown in M9 media are enriched in intracellular-stage proteins.

Fig. 1

a Venn diagram depicting the unique and shared proteins found in OMVs derived from Bp grown in M9 versus LB media. 109 proteins are shown across the two preparations. b Presence of Hcp-1 was confirmed in M9 OMVs by Western blot with rat anti-Hcp-1 sera. Hcp-1 band is shown at 21 kDa. MW molecular ladder. c Large-scale blast score ratio (LS-BSR) for protein presence and conservation among B. pseudomallei. The conservation of proteins in the M9 and LB preparations was compared using tblastn LS-BSR analysis using coding regions from 407 sequenced B. pseudomallei genomes. Protein conservation was determined by blast score ratio cut-offs of ≥0.9 or ≥0.95. The percentage of the 407 tested genomes that contained each protein are shown in the heat map where <90% are shown in white and 100% are shown in red.