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. 2021 Jan 29;4:143. doi: 10.1038/s42003-021-01664-7

Fig. 2. CARTIV promotors show a robust and additive effect with IFNγ and TNFα stimulation in HEK293 cells.

Fig. 2

a Representative FACS plots of HEK293T cells infected using lenti viral vectors with RFP670 under the control of the indicated CARTIV promotor and ZsGreen controlled by the ef1α core promotor. At 72 h following infection, the cells were incubated for 48 h with the indicated cytokines (250 U/mL for each cytokine), harvested and analyzed by flow cytometry. Data shown are ZsGreen-positive, single-discriminated, and DAPI-negative results. b Geometric mean of RFP670 in ZsGreen-positive cells normalized to uninfected cells, showing average of triplicates, error bars indicate standard deviation, A two way ANOVA was performed. c Synergism level calculated by dividing the geometric mean of ZS+ cells stimulated with IFN and TNF by the geometric mean of cells stimulated with IFN or TNF. A one-way ANOVA was performed, error bars indicate standard deviation. Results are from one representative experiment of three full experiments performed (including the G2K2); the G1K0.6, G1K1, and G3K3 were compared in more than eight independent experiments.