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. 2021 Jan 29;4:143. doi: 10.1038/s42003-021-01664-7

Fig. 6. CARTIV promotors allow inducible expression of a Herceptin-based CAR, leading to degranulation against HER2-expressing target cells.

Fig. 6

a Schema of the CAR used. b Representative plot of human primary T cells incubated for 48 h with 250 U/mL of TNFα and for 18 h under hypoxic or normoxic conditions. The cells were then stained using an Erbb2-Fc chimeric protein and analyzed by FACS. Data shown are ZsGreen-positive, single-discriminated, and DAPI-negative results. c %CD107a positive and ZsGreen positive human primary T cell following 48 h with 250 U/mL TNFα and for 18 h under hypoxic conditions and incubation with the indicated target cell line. Percentage of CD107a positive cells is normalized to the total number of ZsGreen positive cells. An ordinary one-way anova was performed to test for significance, Error bars indicate standard deviation. d CD107a expression following incubation with MCF7 cells of ZsGreen positive and negative human primary T cells with and without the indicated stimuli. Results for b are from one representative experiment of four performed; c summarizes 2–6 experiments (target-dependent).