Table 1.

MAUS methyl resonance assignment statistics.

Target	MW (kDa)	PDB ID	Origin/resolution (Å)	Number of methyls	Labeling scheme	Short-range NOE distance	% Unique assignments	% Options ≤ 3 and >1
Hβ2ma	11.9	1JNJ	NMR	35	AILV	6.5	89	11
HLA-A01	45.5	6AT9	X-ray/2.9	87	AILV	7.2	64	30
HLA-A02	44.8	1DUZ	X-ray/1.8	94	AILV	6.0	70	15
MBP	40.7	1DMB	X-ray/1.8	76	MILVproS	6.0	75	21
IL-2	15.4	1M47	X-ray/1.9	61	ILV	6.5	74	23
HNHb	15.7	6O56	NMR/1.9	53	ILV	8.0	89	11
REC2	15.6	4CMP	X-ray/2.6	69	ILV	6.5	59	12
REC3	24.5	4ZT0	X-ray/2.9	85	ILV	8.0	67	28

^aNon-stereospecific.

^bUsed symmetrization and clustering tolerances of 0.1 ppm for ¹³C.