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. 2021 Jan 29;4:146. doi: 10.1038/s42003-020-01636-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the study outline indicating time points of tissue harvest after ischemia/reperfusion (IR) injury and representation of the known cellular composition and function at these time points. b Determination of cell viability by DAPI negativity following dissociation and FACS procedure. c Schematic of the experimental setup to determine the percentage cells that are DAPI− but are nucleated as determined by DRAQ5 positivity. d Heatmap of the cell-to-cell transcriptome similarities (1 − Pearson’s correlation coefficient) of 2201 cells obtained from all conditions combined. Cells are clustered based on transcriptome similarity using k-medoids clustering. e t-Distributed Stochastic Neighbor Embedding (tSNE) plot indicating transcriptome similarities across individual cells. Different colors and numbers highlight the clusters identified by k-medoids clustering in (d). f Table highlighting cell types that were characterized by the clustering analysis. g Bar graph showing the proportion of cells originating from the different condition per cluster. CM cardiomyocytes, FB fibroblasts, MP macrophages, NP neutrophils, EC endothelial cells.