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. 2021 Jan 29;11:2692. doi: 10.1038/s41598-021-82164-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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KAP1 suppresses HIV-1 expression (A–D) Microglial cells were transfected with the pNL4-3 Δ ENV Luc vectors (A,B) or with episomal vector LTR-Luc (C,D) in the presence of an increasing dose of KAP1 (A,C), or with a KAP1 knockdown (shKAP1) (B,D). Luciferase activity was measured 48 h post-transfection and after lysis of the cells. Luciferase values were normalized to those obtained with LTR-Luc or pNL4-3 Δ ENV Luc alone. A t-test was performed on 3 independent experiments P (*P < 0.05, **P < 0.01, ***P < 0.001). (E,F) Overexpressions of KAP1, as well as knockdown efficiencies, were validated by Western blotting. KAP1 expression levels were quantified and presented relatively to β-actin expression, using image J software. Full-length blots are presented in Supplementary Figure 1.