Table 1.
Antibodies and reagents.
| Reagent | Reference |
|---|---|
| Anti-CTIP2 antibody | A300-383A Bethyl |
| Anti-KAP1 antibody | Ab10483 Abcam |
| Anti-Tat antibody | ab43014 Abcam |
| Anti-β-actin antibody | A1978 Sigma-Aldrich |
| Anti-Flag antibody | F3165 Sigma-Aldrich |
| Anti-GFP antibody | 632592 Clontech |
| Anti-α-tubulin antibody | Ab4074 Abcam |
| Anti-UBC9 antibody | 610749 BD Biosciences |
| Anti-HA antibody | 901 501 Biolegend |
| Anti-mouse IgG-HRP | Sc-358914 Santa Cruz |
| Anti-rabbit IgG-HRP | Sc-2004 Santa Cruz |
| Anti-KAP1 ChIP grade antibody | C15410236-100 Diagenode |
| Anti-RNA Pol II ChIP grade antibody | 14958 Cell signaling |
| Anti-H3K9me3 ChIP grade antibody | 1396 Cell signaling |
| Anti-HP1α ChIP grade antibody | 2616 Cell signaling |
| Anti-HP1β ChIP grade antibody | 8676 Cell signaling |
| anti-SP1 ChIP grade antibody | 9389 Cell signaling |
| Rabbit IgG | C15410206 Diagenode |
| Mouse IgG | C15400001-15 Diagenode |
| Reverse PCR | 1708890 Bio-Rad |
| Quantitative PCR | SsoAdvanced Universal SYBR Green Supermix – Bio-Rad |
| Anti-proteases | 000000011873580001 Sigma-Aldrich |
| MG132 | BML-PI102-0025 ENZO Life Sciences |
| Carfilzomib | AG-CR1-3669-M001 Bio-Connect |
| TNFα | 300-01A PeproTech |
| HMBA | 224235 Sigma-Aldrich |
| DMA | 20660 Thermo Scientific |
| Anti-flag immunoprecipitation beads | A-2220 Sigma-Aldrich |
| Protein G magnetic beads | C03010021-220 Diagenode |
| Protein A magnetic beads | C03010020-220 Diagenode |
| Protein A Agarose/Salmon Sperm DNA | 16-157 Sigma-Aldrich |