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. 2021 Jan 29;11:2627. doi: 10.1038/s41598-020-80947-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transit characteristics in middle-aged adults receiving placebo or synbiotic for 30 days. Values are means ± SEM except for d (placebo: n = 14, synbiotic: n = 13). Mixed model ANOVA were performed to compare evolution of stool frequency and consistency (time effect: *p < 0.05). Matched-pairs Wilcoxon signed-rank test was performed for the number of days with abdominal discomfort to compare changes from baseline (*p < 0.05). wk week (wk-2: d-13 to d-7; wk -1: d-6 to d0; wk 1: d1 to d7; wk 2: d8 to d14; wk 3: d15 to d21; wk 4: d22 to d28).