Table 1.
Data obtained from Oxford Nanopore runs both mined and retrieved mitochondrial DNA (see text for protocol information).
| Run | Modification to library preparation | Chemistry | Total # of reads (K) | Total yield (Gb) | mt-reads >500 bp | mtDNA yield (kb) | Mapped molecule 1 mt-reads | Mapped molecule 1 yield (kb) | Mapped molecule 2 mt-reads | Mapped molecule 2 yield (kb) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 10 kb shear | 1D, R9.4, SQK-108 | 165.43 | 0.99 | 7 | 25.31 | 7 | 25.31 | 0 | 0 |
| 2 | 10 kb shear, Trypsin | 1D, R9.4, SQK-108 | 353.58 | 2.06 | 16 | 94.42 | 9 | 43.87 | 5 | 24.54 |
| 3 | 2D | 2D, R9.4, SQK-208 | 18.79 | 0.26 | 10 | 27.83 | 9 | 45.02 | 0 | 0 |
| 4 | 5 kb shear | 1D, R9.4, SQK-108 | 34.64 | 0.31 | 0 | 0 | 0 | 0 | 0 | 0 |
| 5 | Trypsin | 1D, R9.4, SQK-208 | 265.17 | 0.98 | 8 | 46.46 | 6 | 14.16 | 1 | 4.836 |
| 6 | Trypsin | 1D, R9.4, SQK-108 | 18.31 | 0.11 | 1 | 3.43 | 1 | 3.39 | 0 | 0 |
| 7 | Trypsin | 1D, R9.4, LSK109 | 1224.00 | 1.30 | 36 | 74.99 | 31 | 64.60 | 4 | 8.22 |
| 8 | Trypsin | 1D, R9.4, LSK109 | 5149.57 | 3.45 | 80 | 112.71 | 51 | 74.69 | 22 | 26.09 |
| Totals | 7229.48 | 9.46 | 158 | 385.15 | 114 | 271.04 | 32 | 63.69 | ||
Run numbers are described in the text: library preparation methods refer to variations in protocols described in the methods section, chemistry is the type of sequencing run and the version of flow cell used. Run numbers 1–3 are SRA run numbers 1–3, run number 4 is SRA run number 5, run number 5 is SRA run number 6, run number 6 is SRA run number 10, run number 7 is SRA run number 14, and run number 8 is SRA run number 15.