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. 2021 Jan 29;4:144. doi: 10.1038/s42003-021-01652-x

Fig. 3. Exendin-4 ameliorated glucose-inducing DPP4 increase, EMT process, inflammation, and oxidative stress in mesothelial cells.

Fig. 3

a Met-5A cells were induced with or without 25 mM D-glucose (Glu) exposure for 120 h. Under 25 mM D-glucose (Glu) induction, Met-5A cells were treated exendin-4 (Exe4) with 0, 10, or 100 nM concentrations, and then observed cell morphology by microcopy at 200×. Scale bars: 50 μm. b Western blotting was performed to detect the protein levels of DPP4, GLP-1, p-SMAD3, and Snail. c Western blotting was performed to evaluate the protein levels of collagen-I (type I collagen), fibronectin, vimentin, and α-SMA. d Western blotting was performed to analyze the protein levels of p-NF-kB, NOX-1, SOD1, and NQO1. e–g Quantitative results of Fig. 3b, c, and d were shown, respectively. The relative levels were normalized and compared by 0 mM glucose concentration. Data represents the analysis of at least 3 independent experiments and shows mean ± SD. * indicates p-value <0.05; ** represents p-value <0.01; and *** is p-value <0.001.