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. 2021 Jan 25;13:693–706. doi: 10.2147/CMAR.S282806

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© 2021 Liu et al.

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HMGA1 was a downstream target of miR-31-5p. (A) Starbase3.0 showed the binding sites of miR-31-5p in HMGA1 3ʹUTR sequence. (B–E) Dual-luciferase reporter assay (B and C) and RIP assay (D and E) were conducted for the analysis of interaction between miR-31-5p and HMGA1. (F and G) The qRT-PCR and Western blot were adopted for assessing the effects of miR-31-5p inhibition or overexpression on HMGA1 mRNA and protein levels in 786-O and CaKi-1 cells. (H–K) HMGA1 expression was assayed by mRNA level via qRT-PCR and protein level via Western blot in RCC/Normal tissues (H and I) and RCC/HK-2 cells (J and K). *P < 0.05.