Table 3.

Raw RT-ddPCR data on the effects of PNA-a101 on miR-125b-5p, miR-101-3p and microRNA Let-7c-5p. For representative data and the final summary, see Fig. 5. N.a.= not applicable.

Experiment	Treatment	Target	Concentration	Copies/20 ml well	Poisson Conf Max	Poisson Conf Min	Positives	Negatives	Accepted Droplets	Threshold	Treated/Untreated
#1	Ctrl	miR-125b-5p	6.7	134	8.2	5.4	95	16616	16711	4015	n.a.
	PNA-a101	miR-125b-5p	3.8	76	4.9	2.8	52	16181	16233	4015	0.57
	Ctrl	miR-101-3p	7.3	146	8.7	6.0	112	17885	17997	4015	n.a.
	PNA-a101	miR-101-3p	2.0	40	2.9	1.4	29	16630	16659	4015	0.28
	Ctrl	Let-7c-5p	86.1	1722	91.0	81.3	1219	16045	17264	3513	n.a.
	PNA-a101	Let-7c-5p	71.8	1436	76.2	67.4	1010	16048	17058	3513	0.83
#2	Ctrl	miR-125b-5p	3.2	64.2	3.5	2.9	47	17550	17597	4015	n.a.
	PNA-a101	miR-125b-5p	2.1	42.6	2.4	1.9	32	17487	17519	4015	0.66
	Ctrl	miR-101-3p	3.7	73	4.0	3.3	49	15996	16045	4015	n.a.
	PNA-a101	miR-101-3p	0.9	17	1.0	0.7	11	15088	15099	4015	0.23
	Ctrl	Let-7c-5p	37.2	744	38.3	36.2	491	17616	18107	3513	n.a.
	PNA-a101	Let-7c-5p	37.7	754	38.8	36.6	452	16067	16519	3513	1.01
#3	Ctrl	miR-125b-5p	3.2	64.4	3.4	3.0	48	18004	18052	4015	n.a.
	PNA-a101	miR-125b-5p	2.1	41.2	2.1	1.9	29	16649	16678	4015	0.64
	Ctrl	miR-101-3p	3.2	65	3.4	3.1	49	18502	18551	4015	n.a.
	PNA-a101	miR-101-3p	0.9	19	1.0	0.8	14	18384	18398	4015	0.29
	Ctrl	Let-7c-5p	35.2	705	36	34.5	352	16345	16697	3513	n.a.
	PNA-a101	Let-7c-5p	31.9	639	32.6	31.3	353	17535	17888	3513	0.91
#4	Ctrl	miR-125b-5p	4.5	90.4	4.7	4.4	57	16228	16285	4015	n.a.
	PNA-a101	miR-125b-5p	2.9	58.8	3.1	2.8	42	17606	17648	4015	0.65
	Ctrl	miR-101-3p	5.1	102	6.2	4.4	98	17754	17852	4015	n.a.
	PNA-a101	miR-101-3p	1.5	30	2.1	0.9	24	16524	16548	4015	0.31
	Ctrl	Let-7c-5p	75.3	1506	84.8	69.9	1003	16053	17056	3513	n.a.
	PNA-a101	Let-7c-5p	63.4	1268	65.9	59.7	754	15924	16678	3513	0.84