Subject	Biochemistry, Genetics and Molecular Biology
Specific subject area	Molecular Medicine, Pulmonary and Respiratory Medicine, Drug Discovery, Pharmacology
Type of data	Chart Tables Graphs Figures Raw sequencing data
How data were acquired	NGS analysis has been performed using an Illumina NextSeq500 platform (Illumina, FC-404-2005). RT-qPCR reactions have been performed using the CFX96 Touch Real-Time PCR Detection System (BioRad, Hercules, CA, USA).
Data format	Raw and Analyzed
Parameters for data collection	For NGS, small RNA library pools were prepared, quantified and sequenced using Illumina NextSeq500 platform and NextSeq® 500/550 High Output Kit v2 (Illumina, FC-404-2005). The raw base-call data generated have been demultiplexed and converted to FASTQ format. The optimal read depth to analyse the miRNA transcriptome was determined at 10 million reads per sample.
Description of data collection	Human airway epithelial Calu-3 cells were treated with R8-PNA-a101 and R8-PNA-a145 for 72 h. Inhibition of miR-101-3p and miR-145-5p was obtained, associated with increase of CFTR expression. The PNAs were functionalized with an octo-arginine R8 peptide for maximizing cellular uptake. After treatment, the miRNome was analysed in untreated and PNA-treated cells by NGS, and the data obtained validated by RT-qPCR and RT-ddPCR.
Data source location	Department of Life Sciences and Biotechnology, University of Ferrara, Via Fossato di Mortara n.74, 44121 Ferrara, Italy
Data accessibility	Data are available within this article. The NGS raw data are available at the public expression database “European Nucleotide Archive” (ENA) (https://www.ebi.ac.uk/ena/browser/view/PRJEB39141).
Related research articles	E. Fabbri, A. Tamanini, T. Jakova, J. Gasparello, A. Manicardi, R. Corradini, A. Finotti, M. Borgatti, I. Lampronti, S. Munari, M.C. Dechecchi, G. Cabrini and R. Gambari, Treatment of human airway epithelial Calu-3 cells with a Peptide-Nucleic Acid (PNA) targeting the microRNA miR-101-3p is associated with increased expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene, Eur. J. Med. Chem. (2020) https://doi.org/10.1016/j.ejmech.2020.112876. R. Gambari, J. Gasparello, E. Fabbri, M. Borgatti, A. Tamanini, A. Finotti, Peptide Nucleic Acids for MicroRNA Targeting. Methods Mol Biol. 2105 (2020) 199-215. https://doi.org/10.1007/978-1-0716-0243-0_12.