Figure 3.

miR-130a targets AMPKα and AMPKβ. (A) Target seed sequences for miR-130a on PRKAA1 (AMPKα) and PRKAB1 (AMPKβ) are bolded. (B) Reporter assay demonstrated decreased PRKAB1 luciferase activity when miR-130a was added (n = 4 wells per group; ^***P < 0.001 using Student's t-test). (C) Two strains of OFs were transfected with miR-130a mimic. After 8 days, cell lysate was collected, and protein expression was analyzed with western blotting. Relative expression graphs are five different OF strains (^**P < 0.01, ^****P < 0.0001 using Student's t-test).