Figure 3. Effect of CypA on cell apoptosis in H₂O₂-stimulated A549 cells.

(A and B) The Caspase-3 activity of A549 (A) or CaLu-3 (B) cells pretreated with 100, 200, or 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM) for 24 h. (C) Immunoblot analysis of Bax, Bcl-2, Caspase-7, Caspase-3, and PARP-1 in A549 cells pretreated with 100, 200, or 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM) for 24 h. (D–H) Relative quantification analysis of Bax (D), Bcl-2 (E), Caspase-7 (F), Caspase-3 (G), and PARP-1 (H). Data information: The data are shown as the means ± SD (A,B,D–H: n=3). *P<0.05, **P<0.01, and ***P<0.001 (unpaired two-tailed Student’s t-test). The data are representative of at least three independent experiments.