Figure 4. Effect of CypA on the PI3K/Akt/mTOR pathway in H₂O₂-stimulated A549 cells.

(A) Immunoblot analysis of the indicated proteins in A549 cells pretreated with 100, 200, or 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM) for 24 h. (B–D) Relative quantification analysis of p-PI3K/PI3K (B), p-Akt/Akt (C), and p-mTOR/mTOR (D). Data information: The data are shown as the means ± SD (B–D: n=3). *P<0.05, **P<0.01, and ***P<0.001 (unpaired two-tailed Student’s t-test). The data are representative of at least three independent experiments.