Figure 5. The protective effects of CypA on H₂O₂-stimulated A549 were reversed by LY294002.

(A) Cell viability of A549 cells pretreated with 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM), in the presence or absence of LY294006 (10 μM) for 24 h. (B) The ROS production of A549 cells pretreated with 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM), in the presence or absence of LY294006 (10 μM) for 24 h. (C) The Caspase-3 activity of A549 cells pretreated with 500 ng/ml hCypA for 2 h, and then treated with H₂O₂ (200 μM), in the presence or absence of LY294006 (10 μM) for 24 h. Data information: The data are shown as the means ± SD (A–C: n=3); **P<0.01 and ***P<0.001 (unpaired two-tailed Student’s t-test). The data are representative of at least three independent experiments.