Figure 2.

Size-exclusion chromatography analysis on CD39-CD73 bifunctional fusions and the control fusion proteins. Purified proteins (∼10 µg) described in Fig. 1 were analyzed by size-exclusion chromatography by use of Superdex 200 columns, equilibrated with PBS (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 2.7 mM KH₂PO₄, pH 7.2). Gel filtration standard components (Bio-Rad) were vitamin B₁₂ (1.36 kDa), myoglobin (17 kDa), ovalbumin (44 kDa), γ-globulin (158 kDa), and thyroglobulin (670 kDa). PBS, phosphate-buffered saline.