Figure 5.

Core planar cell polarity (PCP) proteins are localized abnormally in Spag6-deficient mice. A and B: whole-mount preparation staining of Frizzled class receptor 6 (FZD6) protein in the organ of Corti of Spag6^+/+ mice (A) and Spag6^−/− mice of either sex (B) at postnatal day 1. The tissues were double stained for FZD6 (white) and phalloidin (actin, green). The FZD6 protein was located at the borders of pillar cells and hair cells near the apical face of the cells in the Spag6^+/+ mice (A2). In the Spag6^−/− mice, the polarized distribution of FZD6 was lost (B2), and the protein was distributed along the entire membrane. A3 and B3 are diagrammatic drawings to show the FZD6 distribution (red) in Spag6^+/+ and Spag6^−/−, respectively. Scale bar: 10 μm.