Table 1.

MST analysis of RSA binding to FcRn or cubilin

	Ligand	Binding Affinity(kD µM)
Cub7,8, pH 7.4	RSA	0.5–1 µM
Cub7,8, pH 7.4	cRSA30min	2–4 µM
Cub7,8, pH 7.4	cRSA2h	∼5 µM
Cub7,8, pH 7.4	cRSA4h	>15 µM
rFcRn, pH 6	RSA	10 µM
rFcRn, pH 6	cRSA30min	19.5 ± 3 µM
rFcRn, pH 6	cRSA2h	∼80–100 µM
rFcRn, pH 6	cRSA4h	>100 µM or ND
rFcRn, pH 7.4	RSA	ND

cRSA, carbamylated rat serum albumin; MST, microscale thermophoresis; ND, not determined; RSA, rat serum albumin.