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. 2020 Oct 7;320(1):L29–L40. doi: 10.1152/ajplung.00173.2020

Figure 2.

Figure 2.

Lysyl oxidase (LOX) promotes extracellular matrix production in vitro and ex vivo. A and B: mRNA were extracted from primary human lung fibroblasts treated with recombinant LOX (LOX) or vehicle (VC) for 48 h. Expression of collagen type1α1 (Col1A1) and fibronectin (FN) was evaluated using real-time PCR. The data were obtained from 10–12 different experiments using fibroblasts from lung tissues of 10–12 different individual normal donors. Values represent means ± SD. Graphical presentation of the data analyzed by paired t test. *P < 0.05. C: human lung tissues were treated with vehicle (VC) or recombinant LOX. Samples were harvested after 72 h for Col1A1 and 96 h for LOX of stimulation. Gene expression levels of Col1A1 and LOX were quantified using qPCR. The data were obtained from 8 to 12 different experiments using lung tissues of 8–12 different individual normal donors. Graphical presentation of the data analyzed by paired t test. Values represent means ± SD. *P < 0.05. D: the levels of Col1A1 and FN in the conditioned culture supernatants from human lung tissues were assessed by immunoblotting. The representative data were obtained from three different experiments using conditioned culture supernatants from lung tissues of three different individual healthy control (HC) donors. E: human lung tissues from healthy donors were treated with recombinant LOX (LOX) or vehicle (VC) for 96 h. The amount of collagen in the lung tissues was evaluated by hydroxyproline assay. The data were obtained from six different experiments using lung tissues of six different individual normal donors. Values represent means ± SD. Graphical presentation of the data analyzed by unpaired t test. **P < 0.01. F: human lung tissues were treated with vehicle (VC), LOX inhibitor BAPN (BAPN), recombinant LOX (LOX), LOX and LOX inhibitor BAPN (LOX + BAPN) for 96 h. The amount of collagen in the lung tissues was evaluated by hydroxyproline assay. The data were obtained from three different experiments using lung tissues of three different individual normal donors. Comparison among three or more groups was performed using ANOVA followed by Bonferroni’s test. Values represent means ± SD. *P < 0.05.