Figure 5.
Patch-clamp measurement of hERG in transfected HEK293 cells exposed to vanilla custard. HEK293 cells were cotransfected with hERG and GFP, and exposed for 24 h to 0.375 puffs/mL vanilla custard e-vapor. A: current traces elicited in response to voltage step protocol (10 mV steps from −60 to 60 mV for 3 s, then steps to −40 mV for 1 s, and hyperpolarized to –120 mV for 0.5 s) in an air control (top) and a VC-exposed cell. B: IV curve of the IKr tail current from air control (n = 8) and VC (n = 7)-exposed cells. *P < 0.05, t test. GFP, green fluorescent protein; VC, vanilla custard.